[Effect of Buzhong Yiqi Decoction on Treg cells in rats with autoimmune thyroiditis through TGF-β/Smad signaling pathway].

SMAD公司 汤剂 自身免疫性甲状腺炎 医学 信号转导 转化生长因子 甲状腺炎 免疫学 癌症研究 传统医学 药理学 内科学 内分泌学 细胞生物学 生物 甲状腺
作者
Ai-Jing Chu,Yuyuan Lu,Jianlin Qiao,Zhongyuan Xia
出处
期刊:PubMed 卷期号:49 (19): 5288-5296
标识
DOI:10.19540/j.cnki.cjcmm.20240712.702
摘要

To investigate the effect of Buzhong Yiqi Decoction on regulatory T cells(Treg) in experimental rats with autoimmune thyroiditis(EAT) through the transforming growth factor-β(TGF-β)/Smad signaling pathway. Female SD rats were immunized with iodine-rich drinking water combined with Freund's adjuvant and porcine thyroglobulin(pTG) to establish the EAT model of rats, and the levels of serum thyroperoxidase antibody(TPOAb) and thyroglobulin antibody(TGAb) were detected. Pathological sections by hematoxylin-eosin(HE) staining were observed. Treg in the rats' spleen were extracted by immunomagnetic beads after the successful modeling and identified by flow cytometry. The extracted Treg were divided into blank group, Buzhong Yiqi Decoction group, TGF-β group, antagonist(LY3200882), and antagonist(LY3200882)+Buzhong Yiqi Decoction group. After the intervention, the cell counting kit-8(CCK-8) experiment was conducted to detect cell viability. Western blot and quantitative real-time PCR(RT-qPCR) were used to detect the expression of TGF-β/Smad signaling pathway-related proteins and genes. The results showed that the levels of TPOAb and TGAb increased in the rats in the model group compared to the rats in the blank group. HE staining showed that part of the follicles in the thyroid tissue of the rats in the model group were destroyed, and a large number of lymphocytes were infiltrated, indicating that the modeling was successful. After Treg were administered in vitro, CCK-8 results showed that the serum concentration of Buzhong Yiqi Decoction was below 40% to promote cell proliferation. The Buzhong Yiqi Decoction-containing serum group could increase the protein expression of TGF-β1, FoxP3, Smad2, and Smad4 compared with the blank serum group, while the expression of p-Smad2, p-Smad3, and Smad3 increased compared with the blank serum group, but the difference was not statistically significant. Compared with the antagonist group, the protein expressions of p-Smad2, Smad2, p-Smad3, Smad3, Smad4, and Smad7 did not significantly increase or decrease in the antagonist group after adding Buzhong Yiqi Decoction-containing serum. RT-qPCR showed that compared with the blank serum group, the mRNA expression of TGF-β1, FoxP3, Smad2, Smad3, Smad4, and Smad7 in the Buzhong Yiqi Decoction group increased or decreased in the same trend as that in the TGF-β group, but there was no statistical significance. After Buzhong Yiqi Decoction-containing serum was added to the antagonist group, the mRNA levels of TGF-β1, FoxP3, Smad2, Smad3, Smad4, and Smad7 were not statistically significant. In conclusion, Buzhong Yiqi Decoction could promote the stability and activity of Treg cells by promoting the secretion of TGF-β1 and regulating the expression of key signaling molecules TGF-β1, Smad2, and Smad4 in the TGF-β/Smad signaling pathway, thus affecting the immune balance of Th17/Treg and inhibiting the inflammatory response of rats with EAT.

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
cherry323完成签到,获得积分10
刚刚
刚刚
1秒前
hahhh7完成签到,获得积分10
1秒前
sun应助黑痴采纳,获得20
1秒前
yibiy完成签到,获得积分10
2秒前
3秒前
qiqi发布了新的文献求助10
4秒前
0h完成签到,获得积分10
4秒前
充电宝应助ljkshr采纳,获得10
4秒前
5秒前
www发布了新的文献求助10
6秒前
7秒前
7秒前
十九完成签到,获得积分10
7秒前
小蘑菇应助XXJ采纳,获得10
7秒前
桕火慢熬完成签到,获得积分10
7秒前
脑洞疼应助开始游戏55采纳,获得10
8秒前
陶陶发布了新的文献求助10
9秒前
xiaoxiaomi应助万木采纳,获得20
9秒前
鱼头关注了科研通微信公众号
9秒前
鱼头关注了科研通微信公众号
10秒前
10秒前
10秒前
言西早发布了新的文献求助20
10秒前
可爱的函函应助猴哥采纳,获得10
10秒前
Pan发布了新的文献求助10
11秒前
科目三应助科研通管家采纳,获得10
11秒前
11秒前
英俊的铭应助科研通管家采纳,获得10
11秒前
充电宝应助科研通管家采纳,获得10
11秒前
星辰大海应助科研通管家采纳,获得10
11秒前
无花果应助科研通管家采纳,获得10
11秒前
思源应助科研通管家采纳,获得10
11秒前
12秒前
12秒前
12秒前
gwd完成签到,获得积分10
13秒前
15秒前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Conference Record, IAS Annual Meeting 1977 820
England and the Discovery of America, 1481-1620 600
Teaching language in context (Third edition) by Derewianka, Beverly; Jones, Pauline 550
電気学会論文誌D(産業応用部門誌), 141 巻, 11 号 510
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3583231
求助须知:如何正确求助?哪些是违规求助? 3152508
关于积分的说明 9492920
捐赠科研通 2854882
什么是DOI,文献DOI怎么找? 1569373
邀请新用户注册赠送积分活动 735182
科研通“疑难数据库(出版商)”最低求助积分说明 721082