Development of a novel triplex-PCR assay for the identification of feline hemoplasma species and survey of hemoplasma species in cats in Türkiye

鉴定(生物学) 生物 医学 内科学 植物
作者
Kürşat Altay,Alparslan Çoşkun,Ufuk Erol,Ömer Faruk Şahin,Sefer TÜRK
出处
期刊:Parasitology International [Elsevier BV]
卷期号:: 102969-102969
标识
DOI:10.1016/j.parint.2024.102969
摘要

Three hemoplasma species, Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum, and Candidatus Mycoplasma turicensis, have been identified in domestic and wild felids. M. haemofelis may cause severe clinical manifestations in domestic cats, whereas others can lead to mild infections. Identification of these pathogens is done using molecular diagnostic tools like conventional-PCR or real-time PCR. However, these have disadvantages, such as the failure to differentiate species or high cost. This study aimed to develop a triplex-PCR method for the diagnosis and discrimination of feline hemoplasma species. Furthermore, it is aimed at providing molecular data on the epidemiology of feline hemoplasma species in Türkiye, where there is a paucity of information on these pathogens. Triplex-PCR primers amplifying the 16S rRNA gene regions of M. haemofelis (1022 bp), Ca. Mycoplasma haemominutum (607 bp), and Ca. Mycoplasma turicensis (456 bp) species were designed and optimized. Moreover, the detection limits of the method were also determined and it was found that the primers could detect 0.001 ng/μL amount of DNA for M. haemofelis, 0.0001 ng/μL for Ca. Mycoplasma haemominutum, and 0.0002 ng/μL for Ca. Mycoplasma turicensis in the sample. 286 cat blood samples obtained from Sivas province were researched for feline hemoplasma species. Feline hemoplasma species were detected in samples of 29 out of 286 cats (10.23 %). Five samples (1.74 %) were infected with only M. haemofelis, whereas 22 (7.69 %) were only infected with Ca. Mycoplasma haemominutum. Two samples (0.69 %) were found to be infected with both M. haemofelis and Ca. Mycoplasma haemominutum. Ca. Mycoplasma turicensis was not detected in this study. A triplex-PCR method that can be used for the identification and species differentiation of feline hemoplasma species in hosts was developed. Moreover, hemoplasma species were found to be circulating in cats in the study area, and it is recommended that veterinarians and animal owners take the necessary precautions to protect themselves and their cats from these pathogens.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
1秒前
李健应助liuzengzhang666采纳,获得10
2秒前
4秒前
4秒前
Georgechan完成签到,获得积分10
6秒前
6秒前
6秒前
Akim应助善良的可乐采纳,获得10
6秒前
6秒前
tls完成签到,获得积分10
7秒前
7秒前
7秒前
孙福禄应助稳重筝采纳,获得10
9秒前
呆二龙完成签到 ,获得积分10
9秒前
七七完成签到,获得积分10
9秒前
YCI完成签到 ,获得积分20
9秒前
lauhoihung完成签到,获得积分10
10秒前
古往今来应助傅全有采纳,获得20
10秒前
树袋发布了新的文献求助10
10秒前
11秒前
11秒前
橘子海发布了新的文献求助10
11秒前
光热效应完成签到 ,获得积分20
11秒前
Hello应助HJJ采纳,获得10
11秒前
量子星尘发布了新的文献求助10
12秒前
obdixp发布了新的文献求助20
12秒前
你快睡吧发布了新的文献求助10
13秒前
华仔应助clairr采纳,获得30
13秒前
传奇3应助温柔映阳采纳,获得10
13秒前
lm发布了新的文献求助10
14秒前
15秒前
15秒前
guozizi发布了新的文献求助10
16秒前
17秒前
18秒前
英姑应助xiaolaohu采纳,获得10
18秒前
SciGPT应助爱学习的孩纸采纳,获得10
18秒前
没有昵称发布了新的文献求助10
19秒前
20秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988827
求助须知:如何正确求助?哪些是违规求助? 3531183
关于积分的说明 11252671
捐赠科研通 3269809
什么是DOI,文献DOI怎么找? 1804780
邀请新用户注册赠送积分活动 881885
科研通“疑难数据库(出版商)”最低求助积分说明 809021