细胞分裂素
生物
细胞生物学
转录因子
磷酸化
基因
信号转导
丝氨酸
突变体
锌指
生物化学
生长素
作者
Dejie Du,Zhaoju Li,Jun Yuan,Fei He,Xiongtao Li,Naijiao Wang,Renhan Li,Wensheng Ke,Dongxue Zhang,Zhaoyan Chen,Zihao Jiang,Yunjie Liu,Lingling Chai,Jie Liu,Zhaorong Hu,Weilong Guo,Peng Huiru,Yingyin Yao,Qixin Sun,Zhongfu Ni,Mingming Xin
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2024-08-30
卷期号:10 (35)
标识
DOI:10.1126/sciadv.adp5541
摘要
Leaves play a crucial role in photosynthesis and respiration, ultimately affecting the final grain yield of crops, including wheat ( Triticum aestivum L.); however, the molecular mechanisms underlying wheat leaf development remain largely unknown. Here, we isolated a narrow-leaf gene, TaWAK2-A , through a map-based cloning strategy. TaWAK2-A encodes a wall-associated kinase (WAK), for which a single Ala-to-Val amino acid substitution reduces the protein stability, leading to a narrow-leaf phenotype in wheat. Further investigation suggests that TaWAK2 directly interacts with and phosphorylates TaNAL1, a trypsin-like serine/cysteine protease. The phosphorylated TaNAL1 is then involved in the degradation of the zinc finger transcription factor TaDST, which acts as a repressor of leaf expansion by activating the expression of the cytokinin oxidase gene TaCKX9 and triggering in vivo cytokinin degradation. Therefore, our findings elucidate a signaling cascade involving TaWAK2-TaNAL1-TaDST that sheds light on the regulation of wheat leaf development.
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