密螺旋体
多路复用
多重聚合酶链反应
单纯疱疹病毒
病毒学
梅毒
HSL和HSV色彩空间
聚合酶链反应
生物
医学
病毒
基因
生物信息学
生物化学
人类免疫缺陷病毒(HIV)
作者
Liufeng Yuan,Deju Xia,Qian Zhou,Wenqi Xu,Sihong Xu,Yue-Ping Yin
标识
DOI:10.1016/j.diagmicrobio.2023.115958
摘要
Multiplex PCR can utilize limited clinical material and is more cost-effective and expected to be used for the detection of Treponema pallidum, herpes simplex virus type 1 and 2 (HSV-1,2). We established a multiplex TP-HSV1-HSV2 Polymerase Chain Reaction (multiplex PCR) targeting the conserved regions of the PolA gene of TP and the UL42 gene of HSV1 and HSV2 to test skin lesions of 115 patients suspected of having TP and HSV1/2 infections. The laboratory sensitivities for all 3 pathogens were 300 copies/mL. The overall clinical sensitivity and specificity in secretion samples for TP were 91.7% and 100%, for HSV1 100% and 98%, and for HSV2 89.7% and 100%, respectively. The method appears superior in patients suspected of early TP infection but negative for nontreponemal antibody testing, and the method is also useful for the differential diagnosis of new skin lesions on genital, perianal, and oral sites of patients with a history of previous syphilis.
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