造血
干细胞
生物
细胞生物学
计算生物学
造血干细胞
细胞
遗传学
作者
Jin Ye,Isabel A. Calvo,Itziar Cenzano,Amaia Vilas,Xabier Martínez-de-Morentin,Miren Lasaga,Diego Alignani,Bruno Paiva,Ana C. Viñado,Patxi San Martín-Úriz,Juan Pablo Romero,Delia Quilez Agreda,Marta Miñana Barrios,Ignacio Sancho,Gabriele Todisco,Luca Malcovati,Núria Planell,Borja Sáez,Jesper Tegnér,Felipe Prósper,David Gomez-Cabrero
出处
期刊:iScience
[Elsevier]
日期:2022-05-01
卷期号:25 (5): 104225-104225
被引量:2
标识
DOI:10.1016/j.isci.2022.104225
摘要
Understanding the regulation of normal and malignant human hematopoiesis requires comprehensive cell atlas of the hematopoietic stem cell (HSC) regulatory microenvironment. Here, we develop a tailored bioinformatic pipeline to integrate public and proprietary single-cell RNA sequencing (scRNA-seq) datasets. As a result, we robustly identify for the first time 14 intermediate cell states and 11 stages of differentiation in the endothelial and mesenchymal BM compartments, respectively. Our data provide the most comprehensive description to date of the murine HSC-regulatory microenvironment and suggest a higher level of specialization of the cellular circuits than previously anticipated. Furthermore, this deep characterization allows inferring conserved features in human, suggesting that the layers of microenvironmental regulation of hematopoiesis may also be shared between species. Our resource and methodology is a stepping-stone toward a comprehensive cell atlas of the BM microenvironment.
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