Combination of hyperlipidemia and 17β‐Estradiol induces TMJOA‐like pathological changes in rats

高脂血症 内分泌学 内科学 软骨 细胞凋亡 软骨细胞 医学 病态的 塞来昔布 NF-κB 化学 解剖 炎症 生物化学 糖尿病
作者
Yan Zhao,Yehua Gan
出处
期刊:Oral Diseases [Wiley]
卷期号:29 (8): 3640-3653 被引量:3
标识
DOI:10.1111/odi.14294
摘要

Abstract Objective We explored whether hyperlipidemia or combination of hyperlipidemia and E2 could induce TMJOA. Materials and Methods Four groups of female rats were treated with normal diet, normal diet with E2, high‐fat diet, and high‐fat diet with E2 (HFD/E2), respectively, to induce TMJOA till 8 weeks. Another three groups were then used for COX2 inhibitor celecoxib to block the induction of TMJOA. Primary condylar chondrocytes were treated with combination of E2, ox‐LDL, and corresponding inhibitors for evaluating expressions of related molecules. Results Condylar cartilage proliferation with plenty of chondrocyte apoptosis and increased staining for LOX1, nuclear NF‐κB, IL‐1β, and COX2 at 4 weeks and decreased condylar cartilage and increased subchondral bone density at 8 weeks were observed only in the HFD/E2 group. Celecoxib significantly alleviated the cartilage proliferation and apoptosis in the HFD/E2 group. Serum ox‐LDL increased in both high‐fat diet groups, while serum IL‐1β increased only in the HFD/E2 group. Combination of E2 and ox‐LDL synergistically induced expressions of LOX1, phosphorylated NF‐κB, IL‐1β, and COX2, while LOX1 inhibitor blocked the induction of phosphorylated NF‐κB, and NF‐κB inhibitor the induction of IL‐1β, and IL‐1β inhibitor the induction of COX2. Conclusion Combination of hyperlipidemia and E2‐induced TMJOA‐like pathological changes through LOX1/NF‐κB/IL‐1β/COX2‐signaling pathway.
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