POS1177 TREATMENT OF GOUTY ARTHRITIS IS ASSOCIATED WITH RESTORING GUT MICROBIOTA AND PROMOTING PRODUCTION OF SHORT CHAIN FATTY ACIDS

Background Although factors initiating the inflammatory response to monosodium urate crystals have been identified, the role of the gut microbiota and their metabolites on gout remain unknown. Objectives This study aimed to investigate changes in both gut microbiota and short chain fatty acids (SCFAs) according to inflammatory states of gout in the same patients. Methods This study enrolled 20 patients with gout in the acute state who had active joints and were followed-up until the recovery state with no active joints. Blood and fecal samples were simultaneously collected within 3 days for each disease state. The stool microbiome was analyzed using 16S rRNA sequencing, and serum SCFAs were measured by gas chromatography-mass spectrometry. Differences in gut microbiome and serum SCFAs were compared between the acute and recovery states. Results Beta diversity of the microbiome was significantly different between the acute and recovery states in terms of weighted UniFrac distance. In the recovery state, Prevotellaceae (p = 0.006) and the genus Prevotella (p = 0.009) were significantly enriched, whereas Enterobacteriaceae (p = 0.019) and its derivative genus Shigella (p = 0.023) were significantly decreased compared to the acute state. Similarly, the levels of acetate was dramatically increased in the recovery state compared to the acute state (p < 0.010). Levels of propionate and butyrate tended to increase but without statistical significance. Conclusion Substantial alterations of bacterial composition with promotion of SCFA formation (especially acetate) were found after treatment in patients with gouty arthritis. References [1]Guo Z, Zhang J, Wang Z et al. Intestinal microbiota distinguish gout patients from healthy humans. Sci Rep 2016;6:20602. Disclosure of Interests None declared