Glutamine promotes antibiotic uptake to kill multidrug-resistant uropathogenic bacteria

微生物学 氨苄西林 生物 鲍曼不动杆菌 谷氨酰胺 抗生素 多重耐药 四环素 抗生素耐药性 大肠杆菌 铜绿假单胞菌 细菌 生物化学 氨基酸 遗传学 基因
作者
Xianliang Zhao,Zhuang‐Gui Chen,Tian‐Ci Yang,Ming Jiang,Jie Wang,Zhi-xue Cheng,Man‐jun Yang,Jiaxin Zhu,Tiantuo Zhang,Hui Li,Bo Peng,Xuan‐xian Peng
出处
期刊:Science Translational Medicine [American Association for the Advancement of Science (AAAS)]
卷期号:13 (625) 被引量:103
标识
DOI:10.1126/scitranslmed.abj0716
摘要

The prevalence of multidrug-resistant bacteria has been increasing rapidly worldwide, a trend that poses great risk to human and animal health and creates urgent need for pharmaceutical and nonpharmaceutical approaches to stop the spread of disease due to antimicrobial resistance. Here, we found that alanine, aspartate, and glutamate metabolism was inactivated, and glutamine was repressed in multidrug-resistant uropathogenic Escherichia coli using a comparative metabolomics approach. Exogenous glutamine promoted β-lactam–, aminoglycoside-, quinolone-, and tetracycline-induced killing of uropathogenic E. coli and potentiated ampicillin to eliminate multidrug-resistant Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella peneumoniae, Edwardsiella tarda, Vibrio alginolyticus, and Vibrio parahaemolyticus. Glutamine-potentiated ampicillin-mediated killing was effective against biofilms of these bacteria in a mouse urinary tract infection model and against systemic infection caused by E. coli, P. aeruginosa, A. baumannii, or K. peneumoniae in a mouse model. Exogenous glutamine stimulated influx of ampicillin, leading to the accumulation of intracellular antibiotic concentrations that exceeded the amount tolerated by the multidrug-resistant bacteria. Furthermore, we demonstrated that exogenous glutamine promoted the biosynthesis of nucleosides including inosine, which in turn interacted with CpxA/CpxR and up-regulated OmpF. We validated the physiological relevance of the mechanism by showing that loss of purF, purH, cpxA, or ompF elevated antibiotic resistance in antibiotic-sensitive strains. In addition, glutamine retarded the development of ampicillin resistance. These results may facilitate future development of effective approaches for preventing or managing chronic, multidrug-resistant bacterial infections, bacterial persistence, and difficult-to-treat bacterial biofilms.
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