化学
赫拉
细胞凋亡
流式细胞术
钌
活性氧
分子生物学
细胞毒性T细胞
癌细胞
MTT法
细胞周期
细胞生长
细胞
体外
生物化学
癌症
生物
催化作用
遗传学
作者
Dan Wan,Shang-Hai Lai,Chuan-Chuan Zeng,Cheng Zhang,Bing Tang,Yun‐Jun Liu
标识
DOI:10.1016/j.jinorgbio.2017.04.026
摘要
Two new ligand PTTP (2-phenoxy-1,4,8,9-tetraazatriphenylene) and FTTP (2-(3-fluoronaphthalen-2-yloxy)-1,4,8,9-tetraazatriphenylene) and their six ruthenium(II) polypyridyl complexes [Ru(N-N)2(PTTP)](ClO4)2 and [Ru(N-N)2(FTTP)](ClO4)2 (N-N = dmb: 4,4′-dimethyl-2,2′-bipiridine; dmp: 2,9-dimethyl-1,10-phenanthroline; ttbpy: 4,4′-ditertiarybutyl-2,2′-bipyridine) were synthesized and characterized. The cytotoxic activity of the complexes against cancer cells HeLa, BEL-7402, A549, HepG-2, HOS and normal cell LO2 was evaluated by MTT method. The IC50 values range from 1.5 ± 0.1 to 55.9 ± 7.5 μM. Complex 3 shows the highest cytotoxic activity toward BEL-7402 cells (IC50 = 1.5 ± 0.1 μM). Complex 5 displays most effective inhibition of the cell growth in A549 and HOS cells with low IC50 values of 2.5 ± 0.6 and 2.6 ± 0.1 μM, respectively. The apoptosis, reactive oxygen species, mitochondrial membrane potential, DNA damage, autophagy and anti-metastasis assay were investigated under a fluorescent microscope. The cell cycle arrest was assayed by flow cytometry, and the expression of caspases and Bcl-2 family proteins was studied by western blot. The results obtained show that the complexes induce apoptosis in BEL-7402 cells through a ROS-mediated mitochondrial dysfunction pathway.
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