过氧化氢
化学
合理设计
部分
荧光
分子内力
生物分子
过氧化物
光化学
组合化学
生物化学
纳米技术
立体化学
有机化学
材料科学
物理
量子力学
作者
Xilei Xie,Xiu’e Yang,Tianhong Wu,Yong Li,Mengmeng Li,Qi Tan,Xu Wang,Bo Tang
标识
DOI:10.1021/acs.analchem.6b01256
摘要
Hydrogen peroxide, an important biomolecule, receives earnest attention because of its physiological and pathological functions. In this Article, we present the rational design, characterization, and biological application of a mitochondria-targetable NIR fluorescent sensor, Mito-NIRHP, for hydrogen peroxide visualization. Mito-NIRHP utilizes a unique reaction switch, α-ketoamide moiety, to turn on a highly specific, sensitive, and rapid fluorescence response toward hydrogen peroxide coupled with the intramolecular charge transfer strategy. Mito-NIRHP is competent to track endogenously produced hydrogen peroxide in both living cells and living animals. In addition, utilizing Mito-NIRHP, overgeneration of hydrogen peroxide during ischemia-reperfusion injury was directly visualized at both cell and organ levels.
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