This investigation examined the impact of organizational culture on job satisfaction and intention to leave the organization through a survey of fitness staff. Organizational culture is commonly known as the values, beliefs and basic assumptions that help guide and coordinate member behaviour. The Cultural Index for Fitness Organizations (CIFO) was developed to measure organizational culture in the fitness industry specifically. Exploratory factor analysis revealed eight factors that represent cultural dimensions common to this context: staff competency, atmosphere, connectedness, formalization, sales, service-equipment, service-programs, and organizational presence. Path analysis was used to examine the relationship among the organizational culture factors, job satisfaction and intention to leave. Results produced a partially mediated model of organizational culture that explained 14.3% of the variance in job satisfaction and 50.3% of the variance with intention to leave the organization. The findings highlight the multidimensionality of organizational culture and its complexity in the fitness industry.