小RNA
物候学
生物
RNA干扰
基因敲除
基因沉默
细胞生物学
体内
德罗沙
功能(生物学)
计算生物学
表型
遗传学
核糖核酸
基因
作者
Bernhard Gentner,Giulia Schira,Alice Giustacchini,Mario Amendola,Brian D. Brown,Maurilio Ponzoni,Luigi Naldini
出处
期刊:Nature Methods
[Springer Nature]
日期:2008-11-30
卷期号:6 (1): 63-66
被引量:288
摘要
To study microRNA function in vivo, the authors optimize lentiviral-driven expression of microRNA target sequences in mice and show dose-dependent inhibition of microRNA-mediated regulation of reporter constructs as well as of natural microRNA targets. With the inhibition of a miR-223, they can phenocopy the knockout of this microRNA. Studying microRNA function in vivo requires genetic strategies to generate loss-of-function phenotypes. We used lentiviral vectors to stably and specifically knock down microRNA by overexpressing microRNA target sequences from polymerase II promoters. These vectors effectively inhibited regulation of reporter constructs and natural microRNA targets. We used bone marrow reconstitution with hematopoietic stem cells stably overexpressing miR-223 target sequence to phenocopy the genetic miR-223 knockout mouse, indicating robust interference of microRNA function in vivo.
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