适体
化学
检出限
纳米技术
线性范围
色谱法
材料科学
遗传学
生物
作者
Xu-Qin Ran,Hai‐Long Qian,Xiu‐Ping Yan
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2021-10-12
卷期号:93 (42): 14287-14292
被引量:30
标识
DOI:10.1021/acs.analchem.1c03396
摘要
Sensitive and precise determination of chloramphenicol (CAP) is of great significance for human health due to its high risk in trace amounts. Solid-state artificial nanochannels are expected to be highly promising sensing devices owing to single-molecule sensitivity, target-specific selectivity, and portability. Herein, we report an aptamer self-assembly-functionalized artificial nanochannel-based sensor for highly sensitive and precise determination of CAP. Aptamer self-assembly (AAs) served as the specific recognition component and were in situ grown on the surface of stable anodic aluminum oxide (AAO) nanochannels to develop an AAs@AAO nanochannel-based sensor. Selective interaction with CAP led to the disassembly of AAs and sensitive current change of AAs@AAO nanochannels, allowing sensitive and precise sensing of CAP in complex food samples. The developed AAs@AAO nanochannel-based sensor showed a wide linear range from 0.32 to 1600 pg. mL-1, low limit of detection (LOD) of 0.1 pg. mL-1, high precision with relative standard deviation of 2.9%, and quantitative recoveries of 93.4-102.2% for CAP in milk, milk powder, and honey samples. This work proposes a versatile nanochannel-based platform for facile, sensitive, and precise sensing of hazardous residues in food samples.
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