Detection and Characterization of Sentinel Lymph Node by Ultrasound Molecular Imaging with B7-H3-Targeted Microbubbles in Orthotopic Breast Cancer Model in Mice

微气泡 医学 前哨淋巴结 生物发光成像 淋巴结 乳腺癌 离体 分子成像 淋巴 病理 体内 转移 超声波 癌症 放射科 内科学 荧光素酶 转染 生物 细胞培养 生物技术 遗传学
作者
Zhongqian Hu,Sunitha V. Bachawal,Xuelin Li,Huaijun Wang,Katheryne E. Wilson,Pan Li,Ramasamy Paulmurugan
出处
期刊:Molecular Imaging and Biology [Springer Science+Business Media]
卷期号:24 (2): 333-340 被引量:10
标识
DOI:10.1007/s11307-021-01680-3
摘要

Purpose Accurate identification and assessment of sentinel lymph node (SLN) using noninvasive imaging methods can play a vital role in tumor staging, surgical planning, and prognostic evaluation. In this study, we assessed the efficacy of B7-H3-targeted molecular-ultrasound imaging for the early SLN detection, and characterization in a mouse model of orthotopic breast cancer. Procedures We established a mouse breast cancer model with lymph node metastasis by injecting MAD-MB 231 cells which were engineered to express firefly luciferase reporter gene into the fat pad of the right 4th mammary gland in female BALB/c nude mice. The sole lymph node (LN) close to the tumor was regarded as the SLN for imaging investigation, which included metastatic and non-metastatic SLNs. The LN in the right 4th mammary gland from normal mice was used as normal control (normal mice LN). The commercially available preclinical streptavidin-coated, perfluorocarbon-containing lipid-shelled microbubbles (VisualSonics, Toronto, Canada) were used to generate B7-H3-targeted microbubbles (MBB7-H3) and control microbubbles (MBControl). Then, ultrasound molecular imaging (USMI) was performed using a high-resolution transducer (MS250; center frequency, 21 MHz; Vevo 2100; VisualSonics, Toronto, Canada) after intravenous injection of microbubbles. Results The SLN was clearly detected and located under conventional (B-mode) and contrast-enhanced ultrasonography with microbubble injection. The metastatic SLNs showed a markedly higher signal from B7-H3-targeted microbubbles (MBB7-H3) compared to the non-metastatic SLNs and normal LNs. The metastatic SLN was further confirmed by ex vivo bioluminescence imaging and eventually verified by histological analysis. Conclusions Our findings suggest the potential value of USMI using B7-H3 targeted microbubbles in breast cancer and establish an effective imaging method for the non-invasive detection and characterization of SLN.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
多多发SCI完成签到,获得积分10
1秒前
zcious完成签到,获得积分10
1秒前
1秒前
cc发布了新的文献求助30
1秒前
Zed关闭了Zed文献求助
1秒前
2秒前
2秒前
fjc发布了新的文献求助10
2秒前
小青完成签到 ,获得积分10
2秒前
3秒前
一枚研究僧完成签到,获得积分0
3秒前
3秒前
guons完成签到 ,获得积分10
3秒前
yrq关注了科研通微信公众号
4秒前
to高坚果发布了新的文献求助10
4秒前
合适的毛豆完成签到,获得积分10
4秒前
顾矜应助Ing采纳,获得10
4秒前
torch132完成签到,获得积分10
4秒前
小二郎应助科研通管家采纳,获得10
4秒前
爆米花应助科研通管家采纳,获得10
4秒前
传奇3应助科研通管家采纳,获得10
4秒前
深情的友易完成签到 ,获得积分10
5秒前
脑洞疼应助科研通管家采纳,获得10
5秒前
科研通AI2S应助科研通管家采纳,获得30
5秒前
在水一方应助科研通管家采纳,获得10
5秒前
顾矜应助科研通管家采纳,获得10
5秒前
风趣初瑶应助科研通管家采纳,获得10
5秒前
领导范儿应助科研通管家采纳,获得10
5秒前
CipherSage应助科研通管家采纳,获得30
5秒前
5秒前
5秒前
5秒前
5秒前
5秒前
mryun完成签到,获得积分10
6秒前
6秒前
tian发布了新的文献求助20
6秒前
6秒前
llllllll发布了新的文献求助10
6秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968964
求助须知:如何正确求助?哪些是违规求助? 3513877
关于积分的说明 11170569
捐赠科研通 3249201
什么是DOI,文献DOI怎么找? 1794692
邀请新用户注册赠送积分活动 875297
科研通“疑难数据库(出版商)”最低求助积分说明 804755