Metabolomics and Transcriptomics Provide Insights into Anthocyanin Biosynthesis in the Developing Grains of Purple Wheat (Triticum aestivum L.)

转录组 非重复序列 花青素 氰化物 芍药苷 生物 生物合成 遗传学 植物 基因表达 生物化学 基因 飞燕草素
作者
Fang Wang,Guangsi Ji,Zhibin Xu,Bo Feng,Qiang Zhou,Xiaoli Fan,Tao Wang
出处
期刊:Journal of Agricultural and Food Chemistry [American Chemical Society]
卷期号:69 (38): 11171-11184 被引量:29
标识
DOI:10.1021/acs.jafc.1c01719
摘要

Purple wheat is thought to have beneficial effects on humans owing to its high anthocyanin content. However, a systematic understanding of the anthocyanin biosynthesis process in developing wheat grain is lacking. Here, the dynamic changes in anthocyanin components and transcripts in the grain of purple wheat ZNM168 at five developmental stages (10, 15, 20, 25, and 30 DAF) were characterized. Compared with other anthocyanins, four components, cyanidin 3-O-rutinoside, cyanidin 3-O-glucoside, cyanidin 3,5-O-diglucoside, and malvidin 3-O-glucoside, were significantly accumulated with grain development. In particular, the considerable accumulation of cyanidin 3-O-rutinoside indicated that it was the pivotal pigment for the purple grain. Transcriptome analysis revealed that the nine differentially expressed genes related to anthocyanin biosynthesis belonged to the BZ1 group, the homologous enzyme encoded by the maize Bronze-1 locus, which may primarily serve to glucosylate anthocyanidins. By constructing a gene coexpression network based on weighted gene coexpression network analysis, the TaBZ1 UniGene (TraesCS1D02G019200) was predicted as a core gene in anthocyanin biosynthesis. In addition, correlation analysis between the metabolites and transcripts suggested that TraesCS2A01G527700 (TaCHS) and TraesCS6B01G006200 (TaANS) were considered critical structural genes in the anthocyanin biosynthesis pathway. This study provides insights to exploit genes pinpointed as genetic engineering targets, thereby breeding anthocyanin-enriched wheat.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
桐桐应助sky采纳,获得10
1秒前
张张发布了新的文献求助10
1秒前
阿木发布了新的文献求助30
1秒前
2秒前
zzt发布了新的文献求助10
2秒前
3秒前
NexusExplorer应助hahahah采纳,获得10
3秒前
万能图书馆应助venjohnson采纳,获得10
3秒前
天真香烟完成签到,获得积分10
4秒前
阜睿发布了新的文献求助10
4秒前
心旷神怡发布了新的文献求助10
4秒前
6秒前
6秒前
岁岁菌完成签到,获得积分10
7秒前
7秒前
小蘑菇应助Lawgh采纳,获得10
7秒前
谦让听筠发布了新的文献求助30
8秒前
呜啦啦发布了新的文献求助10
8秒前
Denny完成签到,获得积分10
8秒前
8秒前
SciGPT应助朴实千万采纳,获得10
8秒前
9秒前
性静H情逸完成签到,获得积分10
9秒前
9秒前
zzt完成签到,获得积分20
10秒前
10秒前
舒适煎蛋完成签到,获得积分10
11秒前
赘婿应助科研狗采纳,获得10
11秒前
12秒前
13秒前
柠檬发布了新的文献求助10
14秒前
高源发布了新的文献求助10
15秒前
汤翔发布了新的文献求助10
15秒前
思源应助当下猫采纳,获得10
15秒前
Denny发布了新的文献求助10
16秒前
乐乐应助陶醉觅夏采纳,获得10
16秒前
D1504009654完成签到,获得积分10
16秒前
17秒前
俭朴依白完成签到,获得积分10
17秒前
三叔应助费费采纳,获得10
18秒前
高分求助中
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
Foreign Policy of the French Second Empire: A Bibliography 500
Chen Hansheng: China’s Last Romantic Revolutionary 500
XAFS for Everyone 500
Classics in Total Synthesis IV 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3144366
求助须知:如何正确求助?哪些是违规求助? 2795962
关于积分的说明 7817099
捐赠科研通 2452017
什么是DOI,文献DOI怎么找? 1304837
科研通“疑难数据库(出版商)”最低求助积分说明 627295
版权声明 601419