雌激素
雌激素受体
克罗恩病
结肠炎
炎症性肠病
病理
医学
免疫组织化学
免疫荧光
肠粘膜
生物
内科学
疾病
免疫学
抗体
乳腺癌
癌症
作者
Damian Jacenik,Marta Zielińska,Sylwia Michlewska,Jakub Fichna,Wanda M. Krajewska
摘要
Crohn's disease is the most diagnosed type of inflammatory bowel disease. Chronic inflammation developing in the intestine leads to peristalsis disorder and damage of intestinal mucosa and seems to be associated with an increased risk of colon neoplastic transformation. Accumulating evidence indicates that estrogens and estrogen receptors affect not only hormone-sensitive tissues, but also other tissues not directly related to estrogens, such as the lungs or colon. Here, we describe the protocol for the successful immunofluorescence staining of estrogen receptors in colon obtained from a murine model of TNBS-induced Crohn's disease. A detailed protocol for the induction of Crohn's disease in mice and intestine preparation is provided as well as a step-by-step immunohistochemical procedure using formalin-fixed paraffin-embedded intestine sections. The described methods are not only useful for estrogen receptor detection and estrogen signaling investigation in vivo but can also be applied to for other proteins which may be involved in the development of colitis.
科研通智能强力驱动
Strongly Powered by AbleSci AI