藻蓝蛋白
孵化
萃取(化学)
节螺藻属
化学
色谱法
食品科学
生物量(生态学)
生物化学
生物
蓝藻
农学
遗传学
细菌
作者
Sahar Akaberi,Damaris Krust,Georg Müller,Wolfgang Frey,C. Gusbeth
标识
DOI:10.1016/j.biortech.2020.123099
摘要
Pulsed electric field (PEF) was conducted for the extraction of proteins/C-Phycocyanins from Arthrospira platensis. The cyanobacterial suspension was treated with 1 µs long pulses at an electric field strength of 40 kV·cm-1 and a treatment energy of 114 kJ·kgsus-1 and 56 kJ·kgsus-1. For benchmarking, additional biomass was processed by high pressure homogenization. Homogeneity of the suspension prior to PEF-treatment influenced the protein/C-phycocyanin extraction efficiency. Stability of C-Phycocyanin during post-PEF incubation time was affected by incubation temperature and pH of the external medium. Biomass concentration severely affect proteins/C-Phycocyanins extraction yield via PEF-treatment. The optimum conditions for extraction of proteins/C-Phycocyanin was obtained at 23 °C while incubating in pH 8-buffer. The energy demand for PEF-treatment amounts to 0.56 MJ·kgdw-1 when processing biomass at 100 gdw·kgsus-1. PEF treatment enhances the protein/C-Phycocyanin extraction yield, thus, it can be suggested as preferential downstream processing method for the production of C-Phycocyanin from A. platensis biomass.
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