生物
核糖核酸
抄写(语言学)
RNA甲基化
DNA
细胞生物学
甲基化
核酸
DNA甲基化
分子生物学
甲基转移酶
基因
遗传学
基因表达
语言学
哲学
作者
Hyun Je Kang,Na Young Cheon,Hyun Park,Gyu Won Jeong,Byeong Jin Ye,Eun Jin Yoo,Jun Ho Lee,Jin‐Hoe Hur,Euna Lee,Hongtae Kim,Kyoo‐young Lee,Soo Youn Choi,Whaseon Lee‐Kwon,Kyungjae Myung,Ja Yil Lee,Hyug Moo Kwon
摘要
Abstract R-loops are three-stranded, RNA–DNA hybrid, nucleic acid structures produced due to inappropriate processing of newly transcribed RNA or transcription-replication collision (TRC). Although R-loops are important for many cellular processes, their accumulation causes genomic instability and malignant diseases, so these structures are tightly regulated. It was recently reported that R-loop accumulation is resolved by methyltransferase-like 3 (METTL3)-mediated m6A RNA methylation under physiological conditions. However, it remains unclear how R-loops in the genome are recognized and induce resolution signals. Here, we demonstrate that tonicity-responsive enhancer binding protein (TonEBP) recognizes R-loops generated by DNA damaging agents such as ultraviolet (UV) or camptothecin (CPT). Single-molecule imaging and biochemical assays reveal that TonEBP preferentially binds a R-loop via both 3D collision and 1D diffusion along DNA in vitro. In addition, we find that TonEBP recruits METTL3 to R-loops through the Rel homology domain (RHD) for m6A RNA methylation. We also show that TonEBP recruits RNaseH1 to R-loops through a METTL3 interaction. Consistent with this, TonEBP or METTL3 depletion increases R-loops and reduces cell survival in the presence of UV or CPT. Collectively, our results reveal an R-loop resolution pathway by TonEBP and m6A RNA methylation by METTL3 and provide new insights into R-loop resolution processes.
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