化学
里贝斯
色谱法
抗氧化剂
没食子酸
串联质谱法
类黄酮
芦丁
高效液相色谱法
质谱法
醋酸
有机化学
生态学
生物
作者
Sa Guo,Mingzhen He,Mi Liu,Wenping Huang,Hui Ouyang,Yulin Feng,Guoyue Zhong,Shilin Yang
出处
期刊:Journal of Chromatographic Science
[Oxford University Press]
日期:2019-10-18
卷期号:58 (3): 241-250
被引量:10
标识
DOI:10.1093/chromsci/bmz097
摘要
Abstract Embelia ribes is a traditional Chinese medicine compound used as a remedy for various diseases. Nevertheless, detailed information regarding its chemical composition is unavailable. Herein, ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was used to characterize the components of E. ribes. A total of 56 compounds, including 16 phenolics, 16 flavonoids, 4 coumarins, 5 fatty acids and 15 other compounds were identified. Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract). Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL). Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract. These findings support the notion that E. ribes is an ideal antioxidant and anti-inflammatory agent.
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