生物素化葡聚糖胺
化学
共轭体系
体内
荧光
纳米技术
聚合物
生物物理学
生物素化
材料科学
生物化学
中枢神经系统
生物
神经科学
有机化学
物理
生物技术
量子力学
作者
Yang Liu,Junjun Liu,Jiayi Zhang,Xiucun Li,Fangsiyu Lin,Nan Zhou,Bai Yang,Laijin Lu
出处
期刊:Biomaterials Science
[The Royal Society of Chemistry]
日期:2019-01-01
卷期号:7 (4): 1574-1583
被引量:26
摘要
The anterograde neuroanatomical tracing technique plays a crucial role in studying and charting the complex interconnections of the nervous system. But there are several major limitations for traditional neural tracers, such as complex immunohistochemical staining procedures, low fluorescence intensity and quick fluorescence quenching. Carbon dots (CDs) as fluorescent bio-probes have been widely used in the biological studies due to their superior optical properties, excellent chemical stability, low toxicity, and easy modifications. In this study, biotinylated dextran amine (BDA) and red fluorescent carbonized polymer dots (CPDs) are successfully conjugated to develop a brand-new generation of fluorescent nano-neural tracers: BDA–CPDs. They are successfully applied as fluorescent probes for in vitro and in vivo bioimaging. In vitro biodistribution of BDA–CPDs shows that they distribute mainly in lysosomes and endoplasmic reticulum. Moreover, two metabolic pathways of BDA–CPDs are found through the investigation of in vivo biodistribution of BDA–CPDs. Furthermore, they can be taken up and anterogradely transported by neurons within the peripheral nervous system of rats. Our results suggest that BDA–CPDs have many advantages over traditional tracers, such as low toxicity, high photoluminescence intensity, excellent photostability and easy procedures. Anterograde pathway tracing with BDA–CPDs is a simple, direct and economical way of studying the connections in the nervous system. Therefore, BDA–CPDs are reliable and valuable fluorescent anterograde neural tracers.
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