生物
阿格林
肠神经系统
神经嵴
细胞生物学
细胞外基质
后肠
分泌物
神经科学
胚胎
内分泌学
遗传学
中肠
受体
幼虫
乙酰胆碱受体
植物
作者
Nándor Nagy,Csilla Barad,Ryo Hotta,Sukhada Bhave,Emily Arciero,Dávid Dóra,Allan M. Goldstein
出处
期刊:Development
[The Company of Biologists]
日期:2018-01-01
被引量:48
摘要
The enteric nervous system arises from neural crest cells that migrate, proliferate, and differentiate into enteric neurons and glia within the intestinal wall. Many extracellular matrix (ECM) components are present in the embryonic gut, but their role in regulating ENS development is largely unknown. Here, we identify heparan sulfate proteoglycan proteins, including collagen 18 (Col18) and agrin, as important regulators of enteric neural crest-derived cell (ENCDC) development. In developing avian hindgut, Col18 is expressed at the ENCDC wavefront, while agrin expression occurs later. Both proteins are normally present around enteric ganglia, but are absent in aganglionic gut. Using chick-mouse intestinal chimeras and enteric neurospheres, we show that vagal- and sacral-derived ENCDCs from both species secrete Col18 and agrin. While glia express Col18 and agrin, enteric neurons only express the latter. Functional studies demonstrate that Col18 is permissive while agrin is strongly inhibitory to ENCDC migration, consistent with the timing of their expression during ENS development. We conclude that ENCDCs govern their own migration by actively remodeling their microenvironment through secretion of ECM proteins.
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