Fermentation of banana resistant starch by bifidobacterium in vitro

By fermentation simulation in vitro, this paper studied bifidobacterium proliferation effect on banana resistant starch, and bifidobacterium fermentation of banana resistant starch. After banana resistant starch simulate digestion in vitro through artificial gastric juice and intestinal juice, vaccinating bifidobacterium in rat feces, using digestion products as substrates for the fermentation in anaerobic conditions, samples were collected at 4, 8, 12, 16, 20 h after fermentation, the p H value, SCFAs and lactic acid were measured respectively. The results showed that when banana starch were added to the medium, bifidobacterium increased 3.51 log cfu/m L. As the extension of fermentation time, p H value gradually reduced, SCFAs and lactic acid increased. After 20 h fermentation, total acid content of SCFAs was 118.52 mmol/L, and lactic acid content was 8.147 mmol/L. The experiment proved that banana resistant starch was conducive to the growth of intestinal bifidobacterium, and could be used by intestinal bacteria, maintaining the acidic environment.