对氧磷
荧光
检出限
生物传感器
材料科学
乙二醇
纳米颗粒
PEG比率
荧光显微镜
蛋白质微阵列
纳米技术
乙酰胆碱酯酶
色谱法
化学
微阵列
酶
有机化学
生物化学
经济
基因表达
物理
基因
量子力学
财务
作者
Minsu Kim,Ji Eon Kwon,Kangwon Lee,Won‐Gun Koh
出处
期刊:Biofabrication
[IOP Publishing]
日期:2018-02-16
卷期号:10 (3): 035002-035002
被引量:21
标识
DOI:10.1088/1758-5090/aab004
摘要
In this study, we developed an enzyme-based miniaturized fluorescence biosensor to detect paraoxon, one of the most well-known neurotoxic organophosphorus compounds. The biosensor was fabricated with poly(ethylene glycol) (PEG) hydrogel microarrays that entrapped acetylcholinesterase (AChE) and quantum dots (QDs) as fluorescence reporters. Metal-enhanced fluorescence (MEF) was utilized to amplify the fluorescence signal, which was achieved by decorating QDs on the surface of silica-coated silver nanoparticles (Ag@Silica). The MEF effects of Ag@Silica were optimized by tuning the thickness of the silica shells, and under the optimized conditions, the fluorescence intensity was shown to be increased 5 fold, compared with the system without MEF. PEG hydrogel microarray entrapping QD-decorated Ag@Silica and AChE was prepared via photopatterning process. The entrapped AChE hydrolyzed paraoxon to produce p-nitrophenol within the hydrogel microstructure, which subsequently quenched the fluorescence of the QDs on the surface of Ag@Silica. The MEF-assisted fluorescence detection resulted in a significant enhancement of paraoxon detection. The detection limit was approximately 1.0 × 10−10 M and 2.0 × 10−7 M for sensing with and without MEF, respectively. The successful integration of a hydrogel microarray system with a microfluidic system was demonstrated to be a potential application for the MEF-based micro-total-analysis-system.
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