Objective: To study the distribution of sub-health and occupational stress as well as their correlation among middle school teachers in Tianjin, then provide evidences for prevention and control of the status of sub-health. Methods: A total of 3 522 middle school teachers from six districts of Tianjin were recruited with stratified cluster sampling strategy for the investigation of Sub-Health Measurement Scale version 1.0 (SHMS V1.0) and Occupational Stress Inventory-Revised Edition (OSI-R) . Results: Detection rate of sub-health status among Tianjin middle school teachers was 58.55%. Men had significantly lower sub-health detection rate (55.19%) than women (59.71%) . Sub-health detection rate increased with age (P<0.05) , the sub-health detection rate among middle school teachers more than 50 years old was the highest (66.84%) . The mean score of OSI-R was 403.18±41.80 with the scores of 176.00±21.05, 103.17±17.53, and 124.02±20.28 for ORQ, PSQ, PRQ, respectively, which showed significantly difference compared with the occupational stress norm of China (P<0.001) . The mean scores of OSI-R, ORQ, PSQ, PRQ in different health status were significantly different (P<0.001) . The partial correlation analysis between the scores of sub-health and occupational stress of middle school teachers showed that the scores of occupational role and personal strain were negatively correlated with the scores of sub-health state (P< 0.001) , while, there was significantly positive correlation between the scores of personal resource and the scores of sub-health state (P<0.001) . Conclusion: Sub-health detection rate of middle school teachers in Tianjin is higher. Effective measures should be taken to appropriately mitigate the occupational stress level of middle school teachers, increase personal resources, and scientific and effective health guidance and education should be strengthened.目的: 了解天津市中学教师的亚健康、职业应激现状及两者之间的相关关系,为干预亚健康向疾病转化提供科学依据。 方法: 采用分层整群随机抽样方法,于2013年4至7月,对天津市6个区县抽取的3 522名中学教师以亚健康评定量表(Sub-Health Measurement Scale version 1.0,SHMS V1.0)和职业应激量表(Occupational Stress Inventory-Revised Edition,OSI-R)进行问卷调查。 结果: 天津市中学教师亚健康检出率为58.55%,其中女性亚健康检出率为59.71%,高于男性(55.19%),差异有统计学意义(P<0.05);亚健康检出率随着年龄的增长而升高(χ(2)(趋势)=9.410,P<0.05),≥50岁组亚健康检出率最高(66.84%)。3 522名调查对象OSI-R原始得分为(403.18±41.80)分,其中职业任务问卷(ORQ)、个体应激反应问卷(PSQ)、个体应对资源问卷(PRQ)原始得分分别为(176.00±21.05)、(103.17±17.53)、(124.02±20.28)分,与中国人群常模比较,差异均有统计学意义(P<0.001)。中学教师不同健康状态下OSI-R各问卷的得分差异有统计学意义(P<0.001)。职业任务得分、个体应激反应得分与亚健康量表得分呈负相关(P<0.001),个体应对资源得分与亚健康量表得分呈正相关(P<0.001)。 结论: 天津市中学教师亚健康检出率较高。相关部门应采取有效措施,适当缓解中学教师的职业应激水平,增加个体应对资源,进行科学有效的健康指导与教育。.