黑色素瘤
病理
免疫组织化学
多路复用
组织学
免疫系统
免疫荧光
淋巴系统
医学
生物
抗体
免疫学
癌症研究
生物信息学
作者
Ileana S. Mauldin,Adela Mahmutovic,Samuel J. Young,Craig L. Slingluff
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 573-587
被引量:8
标识
DOI:10.1007/978-1-0716-1205-7_40
摘要
The presence of tertiary lymphoid structures (TLS) is correlated with prolonged patient survival in a variety of solid cancers, including melanoma. However, few methods have been described that could enable a more comprehensive understanding of the organization and functionality of TLS in solid cancers. In this chapter, we describe multiplex immunohistochemistry and microscopy approaches for identifying, characterizing, and quantifying TLS and intra-tumoral immune infiltrates in melanoma. The described methods are not limited to melanoma alone and could be used to evaluate tertiary lymphoid structures in a wide variety of human cancers.
科研通智能强力驱动
Strongly Powered by AbleSci AI