遗传学
等位基因
互补
基因
生物
表型
突变体
突变
遗传分析
作者
Virginia E. Papaioannou,Richard R. Behringer
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2023-11-06
卷期号:2024 (1): pdb.over107961-pdb.over107961
被引量:4
标识
DOI:10.1101/pdb.over107961
摘要
Certain specialized breeding techniques may come in handy during the analysis of a mutation in order to further understanding of the mutation and its interactions with other genes. Different mutant alleles of the gene in question might be available from other sources or mutations with similar phenotypes could potentially be alleles. This could be determined by complementation testing. In the production of a conditional allele, retention of exogenous DNA in the allele could fortuitously disrupt a regulatory element and thus result in a hypomorphic allele, which can be simply tested by breeding. Mutations in different genes frequently affect the same organ, tissue, or cell type through genetic interactions. Common approaches to investigate and interpret genetic interactions are detailed here for gene families, in which there may be redundancy or genetic compensation of different genes, for genes that constitute different components of a biochemical pathway, for genes with overlapping expression patterns, and for unrelated genes that produce similar mutant phenotypes.
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