无乳链球菌
清脆的
微生物学
菌尿
B组
生物
聚合酶链反应
链球菌
病毒学
医学
泌尿系统
内科学
细菌
基因
遗传学
生物化学
作者
Donghong Yu,Bin Liang,Haipo Xu,Lu Chen,Zhoujie Ye,Zhihui Wu,Xinrui Wang
标识
DOI:10.1186/s12941-023-00558-2
摘要
Abstract Background Streptococcus agalactiae or group B Streptococcus (GBS) is a leading infectious cause of neonatal morbidity and mortality. It is essential to establish a robust method for the rapid and ultra-sensitive detection of GBS in pregnant women with premature rupture of membrane (PROM). Methods This study developed a CRISPR-GBS assay that combined the advantages of the recombinase polymerase amplification (RPA) and CRISPR/Cas12a system for GBS detection. The clinical performance of the CRISPR-GBS assay was assessed using vaginal or cervical swabs that were collected from 179 pregnant women with PROM, compared in parallel to culture-based matrix-assisted laser desorption ionization time-of-flight mass spectrometry (culture-MS) method and real-time quantitative polymerase chain reaction (qPCR) assay. Results The CRISPR-GBS assay can be completed within 35 min and the limit of detection was as low as 5 copies μL −1 . Compared with the culture-MS, the CRISPR-GBS assay demonstrated a sensitivity of 96.64% (144/149, 95% confidence interval [CI] 92.39–98.56%) and a specificity of 100% (30/30, 95% CI 88.65–100%). It also had a high concordance rate of 98.88% with the qPCR assay. Conclusions The established CRISPR-GBS platform can detect GBS in a rapid, accurate, easy-to-operate, and cost-efficient manner. It offered a promising tool for the intrapartum screening of GBS colonization.
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