Smart Health Monitoring: Review of Electrochemical Biosensors for Cortisol Monitoring

Abstract Cortisol, also known as the stress hormone, is a crucial corticosteroid hormone that significantly increases secretion in the human body when facing notable stress. Monitoring cortisol levels is crucial for personal stress management and the diagnosis and treatment of certain diseases. Electrochemical biosensors combine the efficient sensitivity of electrochemical technology with the high specificity of biological recognition processes, making them widely applicable in the analysis of human body fluid components. This work outlines the working mechanism of cortisol electrochemical biosensors, focusing particularly on sensing elements such as antibodies, aptamers, and molecularly imprinted polymers. It provides detailed explanations of the operational principles of these different recognition elements. This work summarizes and evaluates the latest advancements in electrochemical biosensors for detecting cortisol in human body fluids, discussing the influence of different recognition elements on sensor design and electrochemical performance. Subsequently, through a comparative analysis of various sensor performances, the work further discusses the challenges in translating laboratory achievements into practical applications, including enhancing key metrics such as sensor reusability, reproducibility, long‐term stability, continuous monitoring capability, and response time. Finally, it offers insights and recommendations for achieving real‐time, continuous, and long‐term monitoring with cortisol electrochemical biosensors.