Evaluation of Cytotoxicity and Anti-Angiogenesis Induced by Crocetin-Delivering Alginate Hydrogel/Chitosan Nanoparticle Carrier System on Human Ovarian Cancer Cell Line (A2780): An In Vitro Evaluation

In the current study, the anticancer activity of Crocetin-loaded chitosan nanoparticles incorporated into an alginate hydrogel on ovarian cancer cells was investigated. Crocetin was loaded into chitosan nanoparticles via ionotropic gelation method and then incorporated into the alginate hydrogel. Then, various In Vitro experiments were performed to characterize the produced delivery system. The anticancer activity of the developed system was evaluated on A2780 cell line. Results showed that Crocetin-loaded carrier system significantly reduced the viability of ovarian cancer cells via PI3K/AKT/mTOR signaling pathway. Furthermore, the anticancer activity of the developed system was associated with a significant reduction in the expression level of VEGF gene. This study suggests potential use of this system to treat ovarian cancer.