石油
细菌
环境化学
化学
碳氢化合物
微生物降解
生物降解
假单胞菌
污染
微生物种群生物学
生物修复
降级(电信)
环境修复
油泥
污染
环境科学
食品科学
制浆造纸工业
微生物
生物
有机化学
生态学
工程类
电信
遗传学
计算机科学
作者
Yufei Ma,Huan Zhu,Hongwei Liu,Yali Xie,Shouxin Zhang,Cheng Yan,Ruyi Feng,Jianping Zhang,Shengke Yang
标识
DOI:10.1002/clen.202300163
摘要
Abstract Exploring the community characteristics of petroleum degrading bacteria and revealing the types of petroleum metabolites produced by degrading bacteria play an important role in solving the problem of oil pollution. In this study, the bacterial suspension was extracted from the sludge of the sewage treatment plant, and the efficient dominant bacteria were enriched and cultured by microbial screening. The optimal environmental conditions and kinetic behavior of microbial degradation of petroleum hydrocarbons in groundwater were discussed, and the metabolites of microbial degradation of petroleum hydrocarbons were analyzed. The results showed that the optimum degradation conditions of the strain were as follows: bacterial suspension inoculum: 2%, pH: 7, temperature: 30°C, initial concentration of contaminated water sample: 500 mg/L. Under these conditions, the microbial petroleum hydrocarbon degradation efficiency was 84.7% and followed the first‐order kinetics; qualitative and quantitative statistical analysis of the metabolites of the microbial degradation of petroleum hydrocarbons by gas chromatography‐time‐of‐flight mass spectrometry was performed. A total of 10 significantly upregulated products and 12 significantly downregulated products were screened, which were significantly different from the metabolites of the control group. Further microbial community analysis showed that Pseudomonas was dominant in the bacterial suspension, more than 99.5%, which was significantly different from the sludge sample, providing data support for the degradation of petroleum hydrocarbons by Pseudomonas . This study has provided a scientific basis for in situ remediation of petroleum pollution in groundwater.
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