单胺氧化酶
单胺氧化酶B
氧化脱氨基
单胺类神经递质
单胺氧化酶A
化学
多巴胺
药理学
生物化学
血清素
苄胺
酶
医学
内科学
药物化学
受体
作者
Bijo Mathew,Jong‐Min Oh,Della Grace Thomas Parambi,Sachithra Thazhathuveedu Sudevan,Sunil Kumar,Hoon Kim
出处
期刊:Methods in molecular biology
日期:2024-01-01
卷期号:: 329-336
标识
DOI:10.1007/978-1-0716-3662-6_24
摘要
Monoamine oxidase (MAO) catalyzes the oxidative deamination of monoamines with two isoforms, namely, MAO-A and MAO-B, in mitochondrial outer membranes. These two types of MAO-A and MAO-B participate in changes in levels of neurotransmitter such as serotonin (5-hydroxytryptamine) and dopamine. Selective MAO-A inhibitors have been targeted for anti-depression treatment, while selective MAO-B inhibitors are targets of therapeutic agents for Alzheimer’s disease and Parkinson’s disease. For this reason, study on the development of MAO inhibitors has recently become important. Here, we describe methods of MAO activity assay, especially continuous spectrophotometric methods, which give relatively high accuracy. MAO-A and MAO-B can be assayed using kynuramine and benzylamine as substrates, respectively, at 316 nm and 250 nm, respectively, to measure their respective products, 4-hydroxyquinoline and benzaldehyde. Inhibition degree and pattern can be analyzed by using the Lineweaver-Burk and secondary plots in the presence of inhibitor, and reversibility of inhibitor can be determined by using the dialysis method.
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