生物
增强子
基因
龙葵
龙葵
转化酶
基因表达
抄写(语言学)
遗传学
细胞生物学
植物
生物化学
蔗糖
语言学
哲学
作者
Xiaobiao Zhu,Airu Chen,Nathaniel Butler,Zixian Zeng,Haoyang Xin,Lixia Wang,Zhaoyan Lv,Dani Eshel,David S. Douches,Jiming Jiang
标识
DOI:10.1093/plcell/koae050
摘要
Abstract Potato (Solanum tuberosum) is the third most important food crop in the world. Potato tubers must be stored at cold temperatures to minimize sprouting and losses due to disease. However, cold temperatures strongly induce the expression of the potato vacuolar invertase gene (VInv) and cause reducing sugar accumulation. This process, referred to as “cold-induced sweetening”, is a major postharvest problem for the potato industry. We discovered that the cold-induced expression of VInv is controlled by a 200-bp enhancer, VInvIn2En, located in its second intron. We identified several DNA motifs in VInvIn2En that bind transcription factors involved in the plant cold stress response. Mutation of these DNA motifs abolished VInvIn2En function as a transcriptional enhancer. We developed VInvIn2En deletion lines in both diploid and tetraploid potato using clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9 (Cas9)-mediated gene editing. VInv transcription in cold-stored tubers was significantly reduced in the deletion lines. Interestingly, the VInvIn2En sequence is highly conserved among distantly related Solanum species, including tomato (Solanum lycopersicum) and other non-tuber-bearing species. We conclude that the VInv gene as well as the VInvIn2En enhancer have adopted distinct roles in the cold stress response in tubers of tuber-bearing Solanum species.
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