Bioinformatics Analysis of the Phytoene Dehydrogenase Gene in Cabbage (Brassica Oleracea Var. Capitata)

Phytoene dehydrogenase (PDS) is an important enzyme in carotenoid biosynthesis.Here, the Brassica oleracea var.capitata PDS (BocPDS) gene sequences were obtained from Brassica database (BRAD), and preformed for bioinformatics analysis.The BocPDS1 and BocPDS2 genes mapped to chromosomes 3 and 4, and contains an open reading frame of 1,698 bp and 1,692bp that encodes a 565 and 563 amino acid protein, respectively.Subcellular localization predicted both BocPDS1 and BocPDS2 were in the chloroplast.The conserved domain of the BocPDS1 and BocPDS2 proteins is PLN02612.Homology analysis indicates that the levels of identity between BocPDS1 and BocPDS2 were 94%, and the PDS protein is apparently conserved during plant evolution, and BocPDSs are most closely related to Cruciferae plants.The findings of the present study provide a molecular basis for the elucidation of PDS gene function in cabbage.