生物加工
3D生物打印
材料科学
生物医学工程
组织工程
微流控
脐静脉
明胶
挤压
纳米技术
化学
生物化学
医学
冶金
体外
作者
Qingmeng Pi,Sushila Maharjan,Yan Xiang,Xiao Liu,Bijay Singh,Anne Metje van Genderen,Felipe Robledo‐Padilla,Roberto Parra‐Saldívar,Ning Hu,Weitao Jia,Changliang Xu,Jian Kang,Shabir Hassan,Haibo Liu,Xu Hou,Ali Khademhosseini,Yu Shrike Zhang
标识
DOI:10.1002/adma.201706913
摘要
Abstract Despite advances in the bioprinting technology, biofabrication of circumferentially multilayered tubular tissues or organs with cellular heterogeneity, such as blood vessels, trachea, intestine, colon, ureter, and urethra, remains a challenge. Herein, a promising multichannel coaxial extrusion system (MCCES) for microfluidic bioprinting of circumferentially multilayered tubular tissues in a single step, using customized bioinks constituting gelatin methacryloyl, alginate, and eight‐arm poly(ethylene glycol) acrylate with a tripentaerythritol core, is presented. These perfusable cannular constructs can be continuously tuned up from monolayer to triple layers at regular intervals across the length of a bioprinted tube. Using customized bioink and MCCES, bioprinting of several tubular tissue constructs using relevant cell types with adequate biofunctionality including cell viability, proliferation, and differentiation is demonstrated. Specifically, cannular urothelial tissue constructs are bioprinted, using human urothelial cells and human bladder smooth muscle cells, as well as vascular tissue constructs, using human umbilical vein endothelial cells and human smooth muscle cells. These bioprinted cannular tissues can be actively perfused with fluids and nutrients to promote growth and proliferation of the embedded cell types. The fabrication of such tunable and perfusable circumferentially multilayered tissues represents a fundamental step toward creating human cannular tissues.
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