异源的
大肠杆菌
异源表达
膜蛋白
膜
重组DNA
生物
囊泡相关膜蛋白8
蛋白质表达
基因
细胞生物学
细菌
计算生物学
化学
生物化学
遗传学
作者
Peer Depping,María Monserrat Román Lara,Athanasios Kesidis,Roslyn M. Bill,Alice Rothnie,Douglas F. Browning,Alan D. Goddard
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 59-78
被引量:1
标识
DOI:10.1007/978-1-0716-2368-8_4
摘要
Over the decades, the bacterium Escherichia coli (E. coli) has become the cornerstone of recombinant protein production, used for heterologous synthesis of a variety of membrane proteins. Due to its rapid growth to high densities in cheap media, and its ease of manipulation and handling, E. coli is an excellent host cell for a range of membrane protein targets. Furthermore, its genetic tractability allows for a variety of gene constructs to be screened for optimal expression conditions, resulting in relatively high yields of membrane protein in a short amount of time. Here, we describe the general workflow for the production of membrane proteins in E. coli. The protocols we provide show how the gene of interest is modified, transferred to an expression vector and host, and how membrane protein yields can be optimized and analyzed. The examples we illustrate are well suited for scientists who are starting their journey into the world of membrane protein production.
科研通智能强力驱动
Strongly Powered by AbleSci AI