Transposon Mutagenesis

Transposon-based mutagenesis of bacterial genomes is a powerful method to identify genetic elements that control specific phenotypes. The most frequently used transposon tools in Pseudomonas aeruginosa are based either on Himar1 mariner or Tn5 transposases, both of which have been used to generate nonredundant mutant libraries in P. aeruginosa. Here we present a detailed protocol for using Himar1 mariner-based transposon mutagenesis to create mutant libraries in P. aeruginosa.