化学
滴定法
酶抑制
酶
结合常数
活动站点
紧密结合
结合位点
酶分析
生物化学
计算化学
无机化学
电子结构
作者
Petr Kuzmič,Kyle C. Elrod,Lynne Cregar,Steve Sideris,Roopa Rai,James W. Janc
标识
DOI:10.1006/abio.2000.4685
摘要
Active site titration by a reversible tight-binding inhibitor normally depends on prior knowledge of the inhibition constant. Conversely, the determination of tight-binding inhibition constants normally requires prior knowledge of the active enzyme concentration. Often, neither of these quantities is known with sufficient accuracy. This paper describes experimental conditions under which both the enzyme active site concentration and the tight-binding inhibition constant can be determined simultaneously from a single dose-response curve. Representative experimental data are shown for the inhibition of human kallikrein.
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