Background: A study of the diversity, distribution and development of fish eggs and larvae as an integral part of a fishery research programme serves as a valuable aid in the proper management of fisheries. The study is also an essential prerequisite in understanding the spawning biomass of forecasting and trends of production. Information available on diversity of fish eggs from Indian waters is inadequate. The present study was conducted to understand the diversity and distribution of pelagic fish eggs in the nearshore waters of Gulf of Mannar. Methods: The present study conducted at three stations-Mandapam, Thoothukudi and Punnakayal in the waters of Gulf of Mannar, during 2017-2019. A total of 684 ichthyoplankton samples were collected. In the laboratory, the samples were sorted and identified. The diversity was calculated by Shannon-Weiner, Margalef richness index and Pielou’s evenness indices. All the diversity indices were done by using the PRIMER-E software. Result: Our investigation was taken up with objective to assess the status of pelagic fish eggs diversity in the nearshore waters of Gulf of Mannar and found to be more productive. From the diversity study, it is evident that the Punnakayal waters are habitat for a rich diversity of fish species and also a spawning and nursery ground for fishes. Fish eggs diversity is significant for management of fishery resources.