上皮内淋巴细胞
生物
肠上皮
免疫学
免疫系统
细胞生物学
平衡
先天免疫系统
上皮
粘膜免疫
舱室(船)
肠粘膜
粘膜免疫学
免疫
遗传学
医学
地质学
内科学
海洋学
作者
Olivia J. James,Maud Vandereyken,Mahima Swamy
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 141-152
被引量:16
标识
DOI:10.1007/978-1-0716-0338-3_13
摘要
Intestinal intraepithelial lymphocytes (IEL) comprise distinct groups of innate-like and memory T cells that collectively form one of the largest T cell compartments in the body. IEL are located within the intestinal epithelium and are the first immune cells in the gut to interact with the food, microbiota, and pathogens that the gut is continually exposed to. IEL can respond rapidly to external insults to protect the small intestinal epithelium but are also considered regulatory cells that are important to maintain the homeostasis of the gut. However, the mechanisms of IEL activation and their interactions within the epithelium remain largely elusive. Indeed, IEL are not commonly evaluated even in studies of gut immunology, potentially because they are perceived as being difficult to isolate and study. In this protocol, we present a simplified method to isolate IEL from the murine small intestine and provide representative data for flow cytometric analyses of the different IEL subsets. We also outline two procedures for culturing IEL, which can permit functional studies and coculture with epithelial cells. These strategies should make studies of this large but enigmatic T cell compartment more accessible and open up understanding of homeostatic mechanisms in the intestine, and tissue-associated immunity.
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