化学
脂质体
光毒性
细胞毒性
磷脂酰胆碱
酞菁
Zeta电位
活力测定
磷脂酸
生物物理学
光动力疗法
体外
生物化学
有机化学
纳米技术
磷脂
膜
纳米颗粒
材料科学
生物
作者
Paulo Emílio Feuser,Jonathann Corrêa Possato,Rahisa Scussel,Rodrigo Cercená,Pedro Henrique Hermes de Araújo,Ricardo Andrez Machado‐de‐Ávila,Alexandre Gonçalves Dal‐Bó
标识
DOI:10.1142/s1088424621500073
摘要
In this study, zinc phthalocyanine (ZnPc) was encapsulated in liposomes (Phosphatidylcholine (PC) from soybean lecithin (95% phosphatidylcholine, 5% lysophosphatidylcholine), and phosphatidic acid) obtained by a reverse-phase evaporation method. Liposomes were characterized and cytotoxicity and phototoxicity assays were performed using mouse embryo fibroblast (NIH3T3) and human breast cancer (MDAMB231), respectively. ZnPc was successfully encapsulated in liposomes ([Formula: see text]80%), presenting single populations with sizes of [Formula: see text]300 nm and negative zeta potential (-35 to -40 mV). The release profile at different pH presented a biphasic release controlled by the Fickian diffusion mechanism. The cytotoxicity assays carried out on NIH3T3 cells showed that the liposomes provided good protection for ZnPc, and did not affect the viability of non-cancerous cells. In contrast, free ZnPc significantly reduced non-cancerous cell viability at higher concentrations. ZnPc loaded in liposomes ensured a higher phototoxic effect on the MDAMB231 cells at all concentrations tested when exposed to low light dose.
科研通智能强力驱动
Strongly Powered by AbleSci AI