聚硫堇/过氧化氢光致电化学敏感界面的构建及其对葡萄糖氧化酶催化活性的测定

A new photoelectrochemical sensing platform for H2O2 was fabricated by electropolymerization of thionine on ITO electrode. This photosensitive interface was employed as an electron acceptor, and H2O2 proceed from enzymatic catalysis was employed as an electron donor, developing a new determination method for catalytic activity of protein. In the paper, the photoelectrochemical response of poly(thionine) immobilized on ITO electrode to H2O2 was described. The effects of bias voltage, pH of electrolyte and concentration of substrate to the photoelectrochemical response were investigated. The feasibility of method was acknowledged through monitoring the catalytic activity of glucose oxidase. The present method is an economical, speedy, simple and convenient technique to detect the catalytic activity of protein without hydrogen peroxidase.