Nanodiamond‐Mediated Intercellular Transport of Proteins through Membrane Tunneling Nanotubes

纳米技术 纳米金刚石 生物物理学 转运蛋白 内体 HEK 293细胞 细胞内 绿色荧光蛋白 细胞生物学 材料科学 化学 生物 生物化学 钻石 基因 复合材料
作者
Chandra Prakash Epperla,Nitin Mohan,Che‐Wei Chang,Chia‐Chun Chen,Huan‐Cheng Chang
出处
期刊:Small [Wiley]
卷期号:11 (45): 6097-6105 被引量:37
标识
DOI:10.1002/smll.201502089
摘要

Recently discovered tunneling nanotubes (TNTs) are capable of creating intercellular communication pathways through which transport of proteins and other cytoplasmic components occurs. Intercellular transport is related to many diseases and nanotubes are potentially useful as drug‐delivery channels for cancer therapy. Here, we apply fluorescent nanodiamond (FND) as a photostable tracker, as well as a protein carrier, to illustrate the transport events in TNTs of human cells. Proteins, including bovine serum albumin and green fluorescent protein, are first coated on 100‐nm FNDs by physical adsorption and then single‐particle tracking of the bioconjugates in the transient membrane connections is carried out by fluorescence microscopy. Stop‐and‐go and to‐and‐fro motions mediated by molecular motors are found for the active transport of protein‐loaded FNDs trapped in the endosomal vehicles of human embryonic kidney cells (HEK293T). Quantitative analysis of the heterotypical transport between HEK293T and SH‐SY5Y neuroblastoma cells by flow cytometry confirm the formation of open‐ended nanotubes between them, despite that their TNTs differ in structural components. Our results demonstrate the promising applications of this novel carbon‐based nanomaterial for intercellular delivery of biomolecular cargo down to the single‐particle level.

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