Heterogeneity of neutrophils in arterial hypertension

生物 流式细胞术 差速离心 髓系细胞 炎症 细胞外 细胞凋亡 中性粒细胞胞外陷阱 表型 活性氧 细胞外小泡 髓样 细胞生物学 免疫学 细胞内 平衡 分子生物学 生物化学 基因
作者
Doris Cerecedo,Ivette Martínez‐Vieyra,Edgar Oliver López‐Villegas,Arturo Hernández‐Cruz,Arlet Loza-Huerta
出处
期刊:Experimental Cell Research [Elsevier]
卷期号:402 (2): 112577-112577 被引量:8
标识
DOI:10.1016/j.yexcr.2021.112577
摘要

Cellular heterogeneity and diversity are recognized to contribute to the functions of neutrophils under homeostatic and pathological conditions. We previously suggested that the chronic inflammatory responses associated with hypertension (HTN) are related to the participation of different subpopulations of neutrophils. Two populations of neutrophils can be obtained by density gradient centrifugation: normal-density neutrophils (NDN) and low-density neutrophils (LDN). However, the lack of standardized functional protocols has limited phenotypic characterization and functional comparisons of LDN and NDN. Based on their capability to incorporate Na+, maturity and activation stage, we characterized NDN and LDN in blood samples from ten patients with HTN and ten healthy individuals (HI) using flow cytometry. We compared the levels of reactive oxygen species (ROS), generation of neutrophil extracellular traps (NETs) and levels of apoptosis in NDN and LDN. In general, the NDN and LDN subpopulations from patients with HTN exhibited higher levels of sodium influx and ROS, and lower levels of apoptosis than the corresponding NDN and LDN subsets from HI. Transmission electron microscopy revealed NDN and LDN from patients with HTN exhibited alterations to mitochondrial morphology and fewer cytoplasmic granules than the corresponding HI subpopulations. Our results indicate both the NDN and LDN subpopulations enhance the effects of inflammation that contribute to the pathophysiology of HTN. Further detailed studies are required to characterize the events during ontogeny of the myeloid lineage that result in the diverse phenotypic characteristics of each subpopulation of LDN and NDN.
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