RNA序列
计算生物学
鉴定(生物学)
基因
转录组
生物
小桶
生物信息学
微阵列分析技术
基因表达
基因表达谱
作者
Zhongchen Li,Jiheng Hao,Kun Chen,Qunlong Jiang,Peijian Wang,Xiaohui Xing,Jiyue Wang,Yinjiang Zhang,Yilei Xiao,Liyong Zhang
出处
期刊:Aging
[Impact Journals, LLC]
日期:2021-05-11
卷期号:13 (9): 12733-12747
标识
DOI:10.18632/aging.202943
摘要
While acknowledging carotid atherosclerosis (CAS) as a risk factor for ischemic stroke, reports on its pathogenesis are scarce. This study aimed to explore the potential mechanism of CAS through RNA-seq data analysis. Carotid intima tissue samples from CAS patients and healthy subjects were subjected to RNA-seq analysis, which yielded, 1,427 differentially expressed genes (DEGs) related to CAS. Further, enrichment analysis (Gene Ontology, KEGG pathway, and MOCDE analysis) was performed on the DEGs. Hub genes identified via the protein-protein interaction network (PPI) were then analyzed using TRRUST, DisGeNET, PaGenBase, and CMAP databases. Results implicated inflammation and immunity in the pathogenesis of CAS. Also, lung disease was associated with CAS. Hub genes were expressed in multiple diseases, mainly regulated by RELA and NFKB1. Moreover, three small-molecule compounds were found via the CMAP database for management of CAS; hub genes served as potential targets. Collectively, inflammation and immunity are the potential pathological mechanisms of CAS. This study implicates CeForanide, Chenodeoxycholic acid, and 0317956-0000 as potential drug candidates for CAS treatment.
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