阴沟肠杆菌
废水
回流
微生物学
肺炎克雷伯菌
大肠杆菌
生物
环境科学
环境工程
生物化学
机械工程
基因
工程类
入口
作者
Paz Aranega-Bou,Nicholas Ellaby,Matthew J. Ellington,Ginny Moore
出处
期刊:Microorganisms
[MDPI AG]
日期:2021-09-03
卷期号:9 (9): 1868-1868
被引量:6
标识
DOI:10.3390/microorganisms9091868
摘要
Sink waste traps and drains are a reservoir for multi-drug resistant Gram-negative bacteria in the hospital environment. It has been suggested that these bacteria can migrate through hospital plumbing. Hospital waste traps were installed in a laboratory model system where sinks were connected through a common wastewater pipe. Enterobacterales populations were monitored using selective culture, MALDI-TOF identification and antibiotic resistance profiling before and after a wastewater backflow event. When transfer between sinks was suspected, isolates were compared using whole-genome sequencing. Immediately after the wastewater backflow, two KPC-producing Enterobacter cloacae were recovered from a waste trap in which Carbapenemase-producing Enterobacterales (CPE) had not been detected previously. The isolates belonged to ST501 and ST31 and were genetically indistinguishable to those colonising sinks elsewhere in the system. Following inter-sink transfer, KPC-producing E. cloacae ST501 successfully integrated into the microbiome of the recipient sink and was detected in the waste trap water at least five months after the backflow event. Seven weeks and three months after the backflow, other inter-sink transfers involving Escherichia coli ST5295 and KPC-producing E. cloacae ST501 were also observed.
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