齿状回
生物
亚颗粒带
原位杂交
神经科学
基因表达谱
细胞生物学
细胞
电池类型
海马体
基因
基因表达
遗传学
祖细胞
干细胞
室下区
作者
Sheel Shah,Eric Lubeck,Wen Zhou,Long Cai
出处
期刊:Neuron
[Elsevier]
日期:2016-10-01
卷期号:92 (2): 342-357
被引量:608
标识
DOI:10.1016/j.neuron.2016.10.001
摘要
Identifying the spatial organization of tissues at cellular resolution from single-cell gene expression profiles is essential to understanding biological systems. Using an in situ 3D multiplexed imaging method, seqFISH, we identify unique transcriptional states by quantifying and clustering up to 249 genes in 16,958 cells to examine whether the hippocampus is organized into transcriptionally distinct subregions. We identified distinct layers in the dentate gyrus corresponding to the granule cell layer and the subgranular zone and, contrary to previous reports, discovered that distinct subregions within the CA1 and CA3 are composed of unique combinations of cells in different transcriptional states. In addition, we found that the dorsal CA1 is relatively homogeneous at the single cell level, while ventral CA1 is highly heterogeneous. These structures and patterns are observed using different mice and different sets of genes. Together, these results demonstrate the power of seqFISH in transcriptional profiling of complex tissues.
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