Ebselen Optimized the Therapeutic Effects of Silver Nanoparticles for Periodontal Treatment

伊布塞伦 牙龈卟啉单胞菌 格氏链球菌 核梭杆菌 化学 细胞毒性 活性氧 银纳米粒子 超氧化物歧化酶 微生物学 氧化应激 抗菌活性 生物膜 药理学 谷胱甘肽过氧化物酶 体外 生物化学 细菌 生物 材料科学 抗生素 纳米颗粒 纳米技术 链球菌科 遗传学
作者
Ye Liang,Bing Wang,Qing Yu,Weijia Wang,Shaohua Ge,Jinlong Shao
出处
期刊:International Journal of Nanomedicine [Dove Medical Press]
卷期号:Volume 18: 8113-8130 被引量:5
标识
DOI:10.2147/ijn.s434579
摘要

Objective: Silver nanoparticles (AgNPs) possess excellent antibacterial effects on periodontal pathogens, but their clinical application is limited mainly due to their cytotoxicity through inducing oxidative stress in human cells.Ebselen disrupts the reactive oxygen species (ROS) scavenging in bacteria and relieves oxidative stress in mammalian cells.This study aimed to assess the antibacterial and anti-inflammatory effects of AgNPs and ebselen as well as the protective effect of ebselen, to further provide the theoretical basis for their future application in periodontal treatment.Methods: The antibacterial and anti-biofilm effects of the synthesized AgNPs combined with ebselen were assessed on Porphyromonas gingivalis (P.gingivalis), Streptococcus gordonii (S. gordonii), and Fusobacterium nucleatum (F.nucleatum) in planktonic condition and as biofilms.In addition, the intracellular bactericidal efficiency of AgNPs and ebselen was evaluated in P. gingivalis-infected human gingival fibroblasts (HGFs).The cytotoxicity, intracellular ROS levels, and potential antioxidative enzymes were detected in HGFs treated with AgNPs and ebselen.Further, the anti-inflammatory effects were evaluated by in vitro and in vivo experiments.Results: The combination of AgNPs and ebselen showed excellent antibacterial effects against planktonic P. gingivalis and F. nucleatum and synergistic antibiofilm effects on all mono-and multi-species biofilms.In addition, ebselen significantly enhanced the intracellular bactericidal efficiency of AgNPs.Furthermore, ebselen combined with up to 20 μg/mL AgNPs showed no obvious cytotoxicity to HGFs.Evidently, ebselen alleviated the AgNPs-induced ROS by increasing the levels of glutathione and superoxide dismutase 2.Moreover, AgNPs and ebselen together declined the release of P. gingivalis-stimulated inflammatory cytokines both in vitro and in vivo, and reduced alveolar bone resorption effectively.Conclusion: AgNPs combined with ebselen would be an effective adjuvant for periodontal treatment owing to their synergistic antibacterial and anti-inflammatory effects.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
zhuxingxing给zhuxingxing的求助进行了留言
2秒前
调研昵称发布了新的文献求助30
3秒前
4秒前
4秒前
六六完成签到,获得积分20
6秒前
uiwh完成签到,获得积分10
7秒前
7秒前
羽言发布了新的文献求助10
10秒前
李健应助啊呜采纳,获得10
11秒前
庞可心完成签到,获得积分10
11秒前
13秒前
13秒前
17秒前
wanci应助青耕采纳,获得10
18秒前
哈哈哈完成签到,获得积分10
18秒前
所所应助小熊采纳,获得30
18秒前
21秒前
zho应助冯勒采纳,获得10
22秒前
专注的硬币完成签到 ,获得积分10
22秒前
23秒前
yahonyoyoyo发布了新的文献求助10
24秒前
24秒前
24秒前
丘比特应助疲惫的砂糖橘采纳,获得10
25秒前
26秒前
顾矜应助judy007采纳,获得10
26秒前
啊呜发布了新的文献求助10
28秒前
28秒前
浙西南呱呱完成签到,获得积分10
28秒前
29秒前
花生油炒花生米完成签到 ,获得积分10
30秒前
小熊发布了新的文献求助30
31秒前
31秒前
33秒前
itachi发布了新的文献求助10
33秒前
zho应助魔幻的雁采纳,获得10
33秒前
小高同学发布了新的文献求助10
33秒前
明明明明明明明明z完成签到,获得积分10
34秒前
35秒前
艾欧比发布了新的文献求助30
35秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Very-high-order BVD Schemes Using β-variable THINC Method 830
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3247717
求助须知:如何正确求助?哪些是违规求助? 2890987
关于积分的说明 8265743
捐赠科研通 2559230
什么是DOI,文献DOI怎么找? 1388048
科研通“疑难数据库(出版商)”最低求助积分说明 650670
邀请新用户注册赠送积分活动 627571