大肠杆菌
异源的
RNA提取
RNA沉默
生物
质粒
基因沉默
分子生物学
计算生物学
遗传学
基因
核糖核酸
RNA干扰
作者
Saiya Duan,Guangjun Wang
出处
期刊:Methods in molecular biology
日期:2024-01-01
卷期号:: 57-64
被引量:1
标识
DOI:10.1007/978-1-0716-3702-9_9
摘要
Double-stranded RNA (dsRNA) is a valuable tool for reverse genetics research and gene silencing applications. It is also an important management method for pests and diseases in agriculture. It can be synthesized both in vivo and in vitro. The latter presents the drawback of high production cost, the former is less expensive and suitable for scalable production. In general, dsRNAs are obtained in vivo from Escherichia coli heterologous systems that require the IPTG-inducible T7 RNA polymerase. In this report, we describe the construction of an RNAi system for the expression of dsRNA using the HT115 bacterial strain and the L4440 plasmid, and the extraction and identification of dsRNA.
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