Transcriptome analysis provides insights into coumarin biosynthesis in the medicinal plant Angelica dahurica cv. Yubaizhi

香豆素 生物 转录组 小桶 生物合成 基因 欧前胡素 计算生物学 基因表达 遗传学 植物 化学 高效液相色谱法 色谱法
作者
Xiaodong Zhang,Caixia Li,Zhanchao Hao,Yongjiang Liu
出处
期刊:Gene [Elsevier]
卷期号:888: 147757-147757 被引量:1
标识
DOI:10.1016/j.gene.2023.147757
摘要

Angelica dahurica roots have a long history of use in traditional Chinese medicine due to their high coumarin content. To address the increasing demand for these roots, a synthetic biology approach has been proposed. Nevertheless, our comprehension of coumarin biosynthesis and its regulation remains limited. In this study, we utilized Hiseq2500 sequencing to analyze the transcriptomes of A. dahurica at different growth stages while concurrently quantifying coumarin content. Differentially expressed gene (DEG) analysis was employed to identify key genes involved in coumarin and terpenoid backbone biosynthesis. Weighted gene co-expression network analysis (WGCNA) was applied to identify gene modules strongly associated with coumarin content, elucidating the regulatory relationships between transcription factors (TFs) and pathway genes. Furthermore, KEGG enrichment analysis was used to explore essential pathways governing coumarin biosynthesis, with the identification of hub genes. Our results indicated that total coumarin content was highest in the roots, followed by leaves and stems, across all three developmental stages. Transcriptome analysis identified a total of 92,478 genes, among which 215 and 30 genes were implicated in coumarin and terpenoid backbone biosynthesis, respectively. Within the 73 identified gene modules by WGCNA, three modules-namely aquamarine1 (comprising two OMTs, one CSE, one AACT, one HDS, two PSs, one 2OGO, four UGTs, and seven CYP450s), darkmagenta (containing one UGT and one HDR), and navajowhite2 (consisting of one HCT, three UGTs, one CYP71A25, one OMT, one CSE, one HDS, and one PT)-were strongly associated with imperatorin, oxypeucedanin, and isoimperatorin content, respectively. KEGG enrichment analysis highlighted significant enrichment of cytochrome P450, transporter, and ubiquitin system pathways. Moreover, TF-gene regulatory analysis unveiled the complexity of coumarin biosynthesis, with 17 TF families regulating 17 genes in the aquamarine1 module, 8 TF families regulating 2 genes in the darkmagenta module, and 8 TF families regulating 7 genes in the navajowhite2 module. These comprehensive findings provide valuable insights into coumarin biosynthesis in A. dahurica, facilitating future research and potential applications in traditional Chinese medicine and synthetic biology strategies.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
可乐完成签到,获得积分10
1秒前
汉堡包应助mei采纳,获得10
1秒前
个性青枫完成签到,获得积分10
2秒前
纯白色的现代水墨给纯白色的现代水墨的求助进行了留言
3秒前
哈比人linling完成签到,获得积分10
3秒前
cwx完成签到,获得积分10
3秒前
小鱼爱吃肉应助诚心的箴采纳,获得20
3秒前
我是老大应助胡杨树2006采纳,获得10
3秒前
3秒前
糊涂芒果完成签到,获得积分10
4秒前
小马甲应助植保匠人采纳,获得10
5秒前
jiabaoyu完成签到,获得积分10
5秒前
5秒前
富二蛋发布了新的文献求助10
5秒前
嗯哼应助双下巴采纳,获得20
5秒前
沉默襄发布了新的文献求助10
5秒前
Shuey完成签到,获得积分10
6秒前
黑暗里看世界完成签到,获得积分10
6秒前
王花花完成签到,获得积分10
6秒前
科研通AI2S应助wyj采纳,获得10
7秒前
7秒前
沐沐完成签到,获得积分10
7秒前
jane完成签到,获得积分10
7秒前
jasmine完成签到 ,获得积分10
7秒前
彭于彦祖应助15759869988采纳,获得30
7秒前
newgeno2003发布了新的文献求助30
8秒前
8秒前
猪肉发布了新的文献求助10
8秒前
子云完成签到,获得积分10
9秒前
冷静宛海完成签到,获得积分10
9秒前
在九月完成签到 ,获得积分10
9秒前
sptyzl完成签到 ,获得积分10
10秒前
yiyi发布了新的文献求助10
10秒前
愛愛愛愛完成签到,获得积分10
10秒前
风趣谷槐完成签到,获得积分10
10秒前
Ava应助卢人龙采纳,获得10
11秒前
合适凡完成签到,获得积分10
11秒前
飞飞发布了新的文献求助20
11秒前
科研通AI2S应助木华采纳,获得10
11秒前
陈开月发布了新的文献求助10
12秒前
高分求助中
Licensing Deals in Pharmaceuticals 2019-2024 3000
Effect of reactor temperature on FCC yield 2000
Very-high-order BVD Schemes Using β-variable THINC Method 1020
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
Near Infrared Spectra of Origin-defined and Real-world Textiles (NIR-SORT): A spectroscopic and materials characterization dataset for known provenance and post-consumer fabrics 610
Mission to Mao: Us Intelligence and the Chinese Communists in World War II 600
MATLAB在传热学例题中的应用 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3303771
求助须知:如何正确求助?哪些是违规求助? 2937960
关于积分的说明 8485658
捐赠科研通 2611928
什么是DOI,文献DOI怎么找? 1426406
科研通“疑难数据库(出版商)”最低求助积分说明 662619
邀请新用户注册赠送积分活动 647170