细胞内
逆转录酶
素数(序理论)
蛋白质亚单位
化学
细胞生物学
分子生物学
生物
生物化学
核糖核酸
数学
基因
组合数学
作者
Karthikeyan Ponnienselvan,Pengpeng Liu,Thomas Nyalile,Sarah Oikemus,Anya T. Joynt,Karen Kelly,Dongsheng Guo,Zexiang Chen,Jeong Min Lee,Celia A. Schiffer,Charles P. Emerson,Nathan D. Lawson,Jonathan K. Watts,Erik J. Sontheimer,Jeremy Luban,Scot A. Wolfe
标识
DOI:10.1101/2023.10.21.563443
摘要
Prime editing efficiency is modest in cells that are quiescent or slowly proliferating where intracellular dNTP levels are tightly regulated. MMLV-reverse transcriptase - the prime editor polymerase subunit - requires high intracellular dNTPs levels for efficient polymerization. We report that prime editing efficiency in primary cells and in vivo is increased by mutations that enhance the enzymatic properties of MMLV-reverse transcriptase and can be further complemented by targeting SAMHD1 for degradation.
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